Nga Maataapono me nga Tikanga o te Tatari Ine na te Liquid Chromatography

Ko te tikanga wehenga o te chromatography wai e ahu mai ana i te rereketanga o te hononga o nga waahanga o te ranunga mo nga wahanga e rua.

E ai ki nga wahanga rereke rereke, ka wehewehea te chromatography wai ki te chromatography wai-totoka, te chromatography wai-wai me te chromatography wahanga here.Ko te mea e whakamahia nuitia ana ko te chromatography wai-totoka me te hiera silica hei whakakii me te chromatography wahanga here me te microsilica hei matrix.

E ai ki te ahua o te wahanga tuutuu, ka taea te wehea te chromatography wai ki te chromatography tīwae, chromatography pepa me te chromatography paparanga angiangi.E ai ki te kaha adsorption, ka taea te wehea ki te chromatography adsorption, chromatography wehewehe, chromatography whakawhiti katote me te chromatography permeation gel.

I nga tau kua pahure ake nei, kua taapirihia he punaha rerenga wai pehanga teitei ki te punaha chromatography tīwae wai kia tere te rere o te wahanga pūkoro i raro i te pehanga teitei hei whakapai ake i te awenga wehenga, na reira ko te kaha teitei (e mohiotia ana ko te pehanga teitei) te chromatography wai. kua puta.

WAHANGA
01 Te Maataapono o te Tatari Ine o te Chromatography Wai

Ki te ine i runga i te tikanga o te kounga, me nga matū parakore hei paerewa;

Ko te ine ine chromatography wai he tikanga ine nui: ara, ko te nui o te wetewete i roto i te ranunga ka whakatauhia mai i te nui o te tauira paerewa parakore.

WAHANGA
02 Turanga mo te Ine ma te Liquid Chromatography

Ko te nui o te wahanga ine (W) he riterite ki te uara whakautu (A) (te teitei teitei, te waahi tihi ranei), W=f×A.

Tauwehe whakatika ine (f): Ko te riterite tonu o te tauira tatauranga ine, a, ko te tikanga a tinana ko te nui o te waahanga ine e tohuhia ana e te uara whakautu wae (roohanga teitei).

Ka taea te whiwhi i te take whakatika ine mai i te nui o te tauira paerewa me tona uara whakautu.

Inehia te uara whakautu o te waahanga e kore e mohiotia, a ka taea te whiwhi i te nui o te waahanga ma te tauwehe whakatika ine.

WAHANGA
03 Nga kupu noa i roto i te tātari ine

Tauira (tauira): he otinga kei roto he wetewete mo te tātari chromatographic.Kua wehewehea ki nga tauira paerewa me te kore e mohiotia.

Paerewa: He hua parakore me te kukū e mohiotia ana.Tauira kore mohio (kaore e mohio): Ko te ranunga e tika ana kia whakamatauria te kukū.

Tauira taumaha: Ko te pauna taketake o te tauira ka whakamatauria.

Dilution: Ko te take dilution o te tauira e kore e mohiotia.

Waahanga : te tihi chromatographic hei wetewete ine, ara, te wetewete kaore e mohiotia ana te ihirangi.

Te nui o te wae (nui): te ihirangi (te kukū ranei) o te matū hei whakamatautau.

Integerity : Ko te tukanga rorohiko mo te ine i te waahi tihi o te tihi chromatographic e te rorohiko.

Anau whakatikatika: He ananga rarangi o te ihirangi waahanga me te uara whakautu, i whakapumau mai i te nui o te matū paerewa e mohiotia ana, i whakamahia hei whakatau i nga ihirangi kore mohiotia o te tātari.

WAHANGA
04 Te Taatari Ine o te Chromatography Wai

1. Tīpakohia he tikanga chromatographic e tika ana mo te tātari ine:

l Whakapumautia te tihi o te waahanga kua kitea me te whakatutuki i te whakataunga (R) nui atu i te 1.5

l Whakatauhia te rite (te ma) o nga tihi chromatographic o nga waahanga kua whakamatauria

l Whakatauhia te tepe kitenga me te tepe ine o te tikanga;tairongo me te awhe rārangi

2. Whakaturia he ananga whakatika me nga tauira paerewa o nga rereketanga rereke

3. Tirohia te tika me te tika o nga tikanga ine

4. Whakamahia te raupaparorohiko whakahaere chromatography ki te whakatinana i te kohinga tauira, te tukatuka raraunga me te ripoata i nga hua

WAHANGA
05 Te tautuhi i nga tihi ine (kounga)

Tautuhia paitia ia tihi chromatographic hei ine

Tuatahi, whakamahia te tauira paerewa hei whakatau i te wa pupuri (Rt) o te tihi chromatographic hei ine.Ma te whakatairite i te wa pupuri, kimihia te wae e rite ana ki ia tihi chromatographic i te tauira kaore e mohiotia.Ko te tikanga chromatographic qualitative he whakataurite i te wa pupuri ki te tauira paerewa.He iti rawa te paearuhe whakau (kounga)

1. Tikanga taapiri paerewa

2. Whakamahia etahi atu tikanga i te wa ano: etahi atu tikanga chromatographic (hurihia te tikanga, penei: te whakamahi i nga pou chromatographic rereke), etahi atu kaitirotiro (PDA: te whakatairite tuāwhiorangi, te rapu whare pukapuka tūāwhiorangi; MS: te tātari hihinga papatipu, te rapu whare pukapuka tūāwhiorangi)

3. Ko etahi atu taputapu me nga tikanga

WAHANGA
06 Te Whakapumautanga o te Tauritenga Ine Ine

Whakaūhia te riterite o te tihi chromatographic (ma)

Me whakarite kia kotahi anake te wae ine i raro i ia tihi chromatographic

Takina mo te pokanoatanga mai i nga matū whaka-tahi (nga poke)

Nga Tikanga mo te Whakapumautanga o te Tauritenga o te Puke o te Chromatographic (Te Ma)

Te whakatairite i nga Spectrograms me te Photodiode Matrix (PDA) Detector

Te Tohu Maamaa Peak

2996 Te Tikanga Koki Maamaa

Nga tikanga ine e whakamahia ana i te WAHA 07

Tikanga ānau paerewa, wehea ki te tikanga paerewa o waho me te tikanga paerewa o roto:

1. Tikanga paerewa o waho: te nuinga e whakamahia ana i roto i te chromatography wai

He raupapa o nga tauira paerewa o nga kohinga e mohiotia ana i whakaritea ma te whakamahi i nga tauira parakore o nga pūhui hei whakamatautau hei tauira paerewa.ka werohia ki roto i te pou tae noa ki tona uara whakautu (te waahi tihi).
I roto i tetahi awhe, he pai te hononga raina i waenga i te kukū o te tauira paerewa me te uara whakautu, ara ko W= f×A , ka hangaia he pihinga paerewa.

I raro i nga ahuatanga whakamatautau rite tonu, werohia te tauira kaore e mohiotia kia whiwhi i te uara whakautu o te waahanga hei ine.E ai ki te whakarea f e mohiotia ana, ka taea te whiwhi i te kukū o te waahanga hei ine.

Nga painga o te tikanga paerewa o waho:te mahi ngawari me te tatauranga, he tikanga ine nui e whakamahia ana;karekau he hiahia kia kitea, kia whakakorehia ia waahanga;e hiahiatia ana te tauira paerewa;kia rite tonu nga tikanga inenga o te tauira paerewa me te tauira e kore e mohiotia;kia tika te rōrahi werohanga.

Nga ngoikoretanga o te tikanga paerewa o waho:Ko nga tikanga whakamatautau e hiahiatia ana kia teitei, penei i te kaha o te kaitoi, te tere o te rere, me te hanganga o te waahanga waea kaore e taea te whakarereke;ko te rōrahi o ia werohanga kia pai te tukurua.

2. Tikanga paerewa o roto: tika, engari raruraru, te nuinga whakamahia i roto i tikanga paerewa

Ko te nui o te paerewa o roto ka taapirihia ki te paerewa hei hanga i tetahi paerewa whakauru, a kua whakaritea he raupapa o nga paerewa mahi e mohiotia ana.Ko te ōwehenga molar o te paerewa ki te paerewa o roto i te paerewa whakauru ka noho tonu.Werohia ki roto i te tīwae chromatographic ka tango (tauira paerewa taumata teitei/rohe tauira paerewa o roto) hei uara whakautu.E ai ki te hononga raina i waenga i te uara whakautu me te kukū o te paerewa mahi, ara W= f×A , ka hangaia he pihinga paerewa.

Ka taapirihia te nui o te paerewa o roto ki te tauira kaore e mohiotia ana ka werohia ki roto i te pou kia whiwhi ai i te uara whakautu o te waahanga hei ine.E ai ki te whakarea f e mohiotia ana, ka taea te whiwhi i te kukū o te waahanga hei ine.

Nga ahuatanga o te tikanga paerewa o roto:I te wa o te mahi, ka whakakotahihia te tauira me te paerewa o roto ka werohia ki roto i te pou chromatographic, na te mea ko te tauwehenga o te nui o te waahanga ine ki te paerewa o roto i roto i te otinga whakauru ka mau tonu te huringa o te ruri tauira. e kore e pa ki nga hua ine..Ko te tikanga paerewa o roto ka whakakore i te awe o te tauira rōrahi , tae noa ki te waahanga pūkoro me te kaitirotiro, na reira he tika ake i te tikanga paerewa o waho.

WAHANGA
08 Nga mea e pa ana ki nga hua tātari ine

Ko te hapa o te tika ka taea e:

He he te whakaurunga o te waahi tihi, he tauira pirau, he poke ranei i whakaurua i te wa o te whakarite tauira, te ipu tauira kaore i hiritia, te tauira, te whakarewa whakarewa ranei, te takai tauira he, nga raru werohanga tauira, te takahanga paerewa o roto.

Ko nga take pea mo te iti o te tika:

He he te whakaurunga o te tihi, he werohia, he raru werohanga ranei, he tauira pirau, he poke ranei i whakaurua mai i te wa o te whakarite tauira, nga raru chromatographic, te whakautu a te kaitirotiro whakaheke.